Effect of perturbing diacylglycerol metabolism on cytosolic free Ca2+ oscillations induced in single hepatocytes

[摘要]

Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca²⁺-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC₈) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These results agree with a negative feedback effect of protein kinase C on free Ca oscillations.

[学科分类] 生物化学/生物物理

[关键词] Ca2+ oscillation;Diacylglycerol;R 59 022;diC8;Single hepatocyte;free Ca;cytosolic concentration of free Ca2+;DG;sn-1;2-diacylglycerol;diC8;sn-1;2-dioctanoylglycerol