Changes in chromatin structure due to hypomethylation induced with 5‐azacytidine or DL‐ethionine

[摘要]

Changes in chromatin structure of the HRS60 family of repetitive sequences in tobacco DNA were studied after hypomethylation induced with 5-azacytidine or DL-ethionine. The TaqI site in the HRS60 units lies in nucleosomal core regions and its cleavage is enhanced in the hypomethylated chromatin. In contrast, the cleavage of the Sau3A1 site located in linker DNA does not depend on the level of methylation of DNA.

[学科分类] 生物化学/生物物理

[关键词] DNA methylation;Nucleosome positioning;Repetitive DNA sequence;Tobacco tissue culture;AzaC;5-azacytidine;EtBr;ethidium bromide;Ethi;DL-ethionine;MNase;micrococcal nuclease;TE;10 mM Tris-HC1;1 mM EDTA;pH 8