RNase H activity of HIV reverse transcriptases is confined exclusively to the dimeric forms

[摘要]

A method for the rapid preparation of a defined substrate to monitor RNase H activity has been developed. Using this substrate, we have investigated the RNase H activities of the different forms of recombinant HIV-1 and HIV-2 reverse, transcriptase (RT) in detail. As we report here, RNase H activity is associated only with the dimeric forms (p51/p66 or p66/p66) of the enzymes

[学科分类] 生物化学/生物物理

[关键词] HIV;Reverse transcriptase;RNase H;Dimerization;BSA;bovine serum albumine;E. coli;Escherichia coli;FPLC;fast protein liquid chromatography;HIV;human immunodeficiency virus;HPLC;high-performance liquid chromatography;PCR;polymerase chain reaction;RT;reverse transcriptase