Inhibition by spermine of the inner membrane permeability transition of isolated rat heart mitochondria

[摘要]

The effect of spermine on the permeability transition of the inner mitochondrial membrane of isolated rat heart mitochondria was evaluated. The permeability transition was triggered using a series of agents (t-butyl hydroperoxide, phenylarsine oxide, carboxyatractylate, and elevated Ca²⁺ and inorganic phosphate concentrations), and was monitored via Ca²⁺-release, mitochondrial swelling and pyridine nucleotide oxidation. By all three criteria, spermine inhibited the transition. A C₅₀ of 0.38 ± 0.06 (SD) mM was measured for inhibition.

[学科分类] 生物化学/生物物理

[关键词] Spermine;Mitochondria;Permeability transition;Ca2+;Swelling;BSA;bovine serum albumin;fraction V;CAT;carboxyatractylate;CsA;cyclosporin A;DMSO;dimethyl sulfoxide;EGTA;ethylene glycol bis(β-aminoethyl ether)-N;N;N′;N′-tetracetic acid;HEPES;4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid;i.m.;inner membrane;MOPS;3-(N-morpholino)propane-sulfonic acid;PhAsO;phenylarsine oxide;Pi;inorganic phosphate;t-BH;t-butyl hydroperoxide