Anticodon‐dependent aminoacylation of RNA minisubstrate by lysyl‐tRNA synthetase

[摘要]

Specific inhibition of mammalian lysyl-tRNA synthetase by polyU is shown. Inhibition of the enzyme is dependent on the length of the oligonucleotide, since oligoU molecules with a length of less than 8 residues do not inhibit the aminoacylation, whilst the effect of oligoU molecules with a length of about 30 residues is the same as that of polyU. Inhibition is a result of recognition by the enzyme of the tRNA^Lys anticodon sequence (UUU) coded by polyU. Aminoacylation of the oligoU molecule with attached CCA sequence (G(U)₂₀-CCA) by yeast and mammalian lysyl-tRNA synthetases is demonstrated.

[学科分类] 生物化学/生物物理

[关键词] Lysyl-tRNA synthetase;RNA-minisubstrate;tRNA recognition