A selective inhibitor of cyclic AMP‐dependent protein kinase, N‐[2‐bromocinnamyl(amino)ethyl]‐5‐isoquinolinesulfonamide (H‐89), inhibits phosphatidylcholine biosynthesis in HeLa cells

[摘要]

In this study, we report that the potent and selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide (H-89) interferes with the incorporation of choline into phosphatidylcholine in HeLa cells. Treatment of cells with 10 μM H-89 for 1 h decreases the phosphatidylcholine biosynthesis by 50%. This inhibition is prevented by simultaneous addition of 10 μM forskolin, while the choline uptake itself is not affected by H-89.

[学科分类] 生物化学/生物物理

[关键词] Phosphatidylcholine biosynthesis;cAMP-dependent protein kinase;N-[2-Bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide (M-89);Forskolin;HeLa cell;PC;phosphatidylcholine;H-89;N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide;TPA;12-tetradecanoyl-phorbol-13-acetate;PBS;phosphate-buffered saline