Multiple Ca2+ signaling pathways converge on CaM kinase in PC12 cells

[摘要]

The role of multifunctional Ca²⁺/calmodulin-dependent protein kinase (CaM kinase) in mediating various Ca²⁺ signaling pathways was examined in PC12 cells. Conversion of the kinase to a Ca²⁺-independent form was used to monitor which neurotransmitters activate the enzyme in situ. CaM kinase responds to Ca²⁺ influx elicited by ligand-gated Ca²⁺ channels for ATP and acetylcholine. It also responds to Ca²⁺ mobilization ofIP₃-sensitive stores elicited by phospholipase C-linked receptors for ATP and acetylcholine as well as by caffeine. CaM kinase mediates the actions of many neurotransmitters and Ca²⁺ signaling pathways.

[学科分类] 生物化学/生物物理

[关键词] Ca2+/calmodulin-dependent protein kinase (CaM kinase);Calcium store;ATP receptor;Muscarinic receptor