Tetrahydro‐6‐biopterin is associated with tetrahydro‐7‐biopterin in primary murine mast cells

[摘要]

Murine bone marrow-derived mast cells proliferate in response to interleukin 3. In addition to 6-biopterin, 7-biopterin was identified in these cells by HPLC analysis of iodine oxidized extracts and by alkaline permanganate oxidation to the 6- and 7-carboxylic acids. 7-Biopterin comprised 31.9 (± 7.7)% of the total biopterin. It was absent in cells which were grown with of l-p-chlorophenylalanine, an inhibitor of tryptophan 5-monooxygenase. Both 6- and 7-biopterin were present in the cell as their tetrahydro forms. From these data we conclude that 7-biopterin, in contrast to e.g. brain tissue, regularly occurs as a normal metabolite in primary mast cells and that it is generated during hydroxylation of tryptophan.

[学科分类] 生物化学/生物物理

[关键词] Mast cell;Tetrahydro-6-biopterin;Tetrahydro-7-biopterin;6-Biopterin;7-Biopterin;Tryptophan 5-mono-oxygenase;H4-6- or 7-biopterin;6- or 7-(l-erythro-1′;2′-dihydroxy-propyl)-5;6;7;8-tetrahydropterin;6- or 7-biopterin;6- or 7-(l-erythro-1′;2′-dihydroxypropyl)-pterin;6- or 7-neopterin;6- or 7-(l-erythro-1′;2′;3′-trihydroxypropyl)-pterin;monapterin;6-(l-threo-1′;2′;3′-trihydroxypropyl)-pterin;6-pyruvoyl-H4pterin;6-pyruvoyl-5;6;7;8-tetrahydropterin;IL-3;interleukin 3