Cloning and sequence analysis of cDNA for rat corticotropin‐releasing factor precursor

[摘要]

DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.

[学科分类] 生物化学/生物物理

[关键词] Corticotropin-releasing factor;Hormone precursor;cDNA cloning;Nucleotide sequence;CRF;corticotropin-releasing factor;prepro-CRF;corticotropin-releasing factor precursor