Purification and characterization of endonucleases DraII and III from Deinococcus radiophilus

[摘要]

Deinococcus radiophilus strain ATCC 27603 contains, apart from endonuclease DraI [1], two additional sequence-specific endonucleases. These enzymes, designated DraII and DraIII, recognize nucleotide sequences with novel specificities, PuG↓GNCCPy and CACNN↓GTG, respectively.

[学科分类] 生物化学/生物物理

[关键词] Restriction enzyme;Nucleotide sequence;Ad;adenovirus;FPLC;fast protein liquid chromatography;Pu;purine;Py;pyrimidine;mec;gene for deoxycytidylate methyl transferase