Fibronectin is a binding partner for the myelin‐associated glycoprotein (siglec‐4a)

[摘要]

The myelin-associated glycoprotein (MAG) mediates cell–cell interactions between myelinating glial cells and neurons. Here we describe the extracellular matrix glycoprotein fibronectin as a binding partner of MAG. It has been identified by affinity precipitation with MAG-Fc from NG108-15 cells and by microsequencing of two peptides derived from a 210-kDa protein band. Western blot analysis showed that fibronectin is also present in MAG binding partners isolated from N₂A (murine neuroblastoma) cells, rat brain and rat spinal cord. Different fibronectin isoforms have been isolated from brains of young and adult rats, indicating that the expression of MAG binding fibronectin changes during development.

[学科分类] 生物化学/生物物理

[关键词] Myelin-associated glycoprotein;Siglec;Sialic acid;Cellular interaction;Extracellular matrix;Fibronectin;ECM;extracellular matrix;FCS;fetal calf serum;Fn;fibronectin;MAG;myelin-associated glycoprotein;MAG-Fc;recombinant extracellular domains of MAG fused to the Fc fragment of human immunoglobulin