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Cyclosporin A increases basal intracellular calcium and calcium responses to endothelin and vasopressin in human coronary myocytes
[摘要]

Cyclosporin A (CsA) is a widely used immunosuppressive agent with severe side effects including hypertension. Here, we investigated the effects of CsA on intracellular free calcium ([Ca2+]i) and the mechanisms involved in vasoconstriction in cultured human coronary myocytes. We used the Fura-2 technique for Ca2+ imaging. Acute application of CsA at therapeutic concentrations (0.1–10 μmol/l) had no effect. Chronic exposure to CsA (1 μmol/l) for 24 h induced a small (20 nmol/l) but highly significant increase of basal [Ca2+]i and enhanced the occurrence of spontaneous Ca2+ oscillations. Endothelin- and vasopressin-induced rises of [Ca2+]i were also enhanced. The demonstration that CsA increases basal [Ca2+]i in addition to its impact on agonist receptor stimulation is of major importance for new therapeutic approaches.

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[效力级别]  [学科分类] 生物化学/生物物理
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