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Regulation of a mammalian Shaker‐related potassium channel, hKv1.5, by extracellular potassium and pH
[摘要]

Using the whole-cell recording mode of the patch-clamp technique we studied the effects of removal of extracellular potassium, [K+]o, on a mammalian Shaker-related K+ channel, hKv1.5. In the absence of [K+]o, current through hKv1.5 was similar to currents obtained in the presence of 4.5 mM [K+]o. This observation was not expected as earlier results had suggested that either positively charged residues or the presence of a nitrogen-containing residue at the external TEA+ binding site (R487 in hKv1.5) caused current loss upon removal of [K+]o. However, the current loss in hKv1.5 was observed when the extracellular pH, pHo, was reduced from 7.4 to 6.0, a behavior similar to that observed previously for current through mKv1.3 with a histidine at the equivalent position (H404). These observations suggested that the charge at R487 in hKv1.5 channels was influenced by other amino acids in the vicinity. Replacement of a histidine at position 463 in hKv1.5 by glycine confirmed this hypothesis making this H463G mutant channel sensitive to removal of [K+]o even at pHo 7.4. We conclude that the protonation of H463 at pH 7.4 might induce a pK a shift of R487 that influences the effective charge at this position leading to a not fully protonated arginine. Furthermore, we assume that the charge at position 487 in hKv1.5 can directly or indirectly disturb the occupation of a K+ binding site within the channel pore possibly by electrostatic interaction. This in turn might interfere with the concerted transition of K+ ions resulting in a loss of K+ conduction.

[发布日期]  [发布机构] 
[效力级别]  [学科分类] 生物化学/生物物理
[关键词] Shaker-related K+ channel;Patch clamp technique;K+ dependence;pH dependence [时效性] 
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