Corticosterone‐induced rapid phosphorylation of p38 and JNK mitogen‐activated protein kinases in PC12 cells

[摘要]

The present study showed that corticosterone (B) could induce a rapid activation of p38 and c-Jun NH₂-terminal protein kinase (JNK) in PC12 cells. The dose–response and time–response curves were bell-shaped with maximal activation at 10⁻⁹ M and at 15 min. RU38486 had no effect, and bovine serum albumin-coupled B could induce the activation. Genistein failed to block the phosphorylation, suggesting the pathway was not involved in tyrosine kinase activity. Phorbol 12-myristate 13-acetate could mimic, while Gö6976 could abolish the actions. These results demonstrated that B might act via a putative membrane receptor to activate p38 and JNK rapidly through a protein kinase C-dependent pathway.

[学科分类] 生物化学/生物物理

[关键词] Corticosterone;Non-genomic;p38;c-Jun NH2-terminal protein kinase;Protein kinase C;PC12 cell;B;corticosterone;B-BSA;bovine serum albumin-coupled corticosterone;MAPK;mitogen-activated protein kinase;Erk;extracellular signal-regulated kinase;JNK;c-Jun NH2-terminal protein kinase;PKC;protein kinase C;PKA;protein kinase A;PMA;phorbol 12-myristate 13-acetate;DMEM;Dulbecco's modified Eagle's medium;NGF;nerve growth factor;RTK;receptor tyrosine kinase