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Sequence‐specific binding property of Arabidopsis thaliana telomeric DNA binding protein 1 (AtTBP1)
[摘要]

We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a single Myb telomeric DNA binding domain at the C-terminus and an extensive homology with other known telomere-binding proteins. The isolated C-terminus of AtTBP1 is capable of sequence-specific DNA binding to plant duplex telomeric DNA. These results suggest that AtTBP1 may play important roles in plant telomere function in vivo.

[发布日期]  [发布机构] 
[效力级别]  [学科分类] 生物化学/生物物理
[关键词] Telomere-binding protein;Telomere;Electrophoresis mobility shift assay;Arabidopsis thaliana;AtTBP1;Arabidopsis thaliana telomeric DNA-binding protein 1;TBP;telomere-binding protein;SMTBD;single Myb telomeric DNA-binding domain;EMSA;electrophoresis mobility shift assay;GST;glutathione S-transferase;TAS;telomere-associated sequences [时效性] 
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