DNA methylation in Trypanosoma cruzi

[摘要]

DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N⁶-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.

[学科分类] 生物化学/生物物理

[关键词] DNA methylation;5-Methylcytosine;Base composition;Trypanosoma cruzi;m5Cyt;5-methylcytosine;m6Ade;N6-methyladenine;HPLC;high-pressure liquid chromatography;PBS;phosphatebuffered saline;EDTA;ethylenediamine-tetraacetic acid;NDNA;nuclear DNA;kDNA;kinetoplast DNA;SDS;sodium dodecyl sulphate;5-aza-C;5-azacytidine