Inactivation of Ca2+‐, Na+K+‐, and H+K+‐ATPases with a carbodiimide derivative of ATP
[摘要] The αmma;P adduct of ATP with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (ATP-EDC) was synthesized and incubated with the Ca-ATPase of sarcoplasmic reticulum with the result that time-dependent complete loss of the enzyme's activity occurred. The inactivation required calcium and magnesium while ATP had a protective effect. ATP-EDC incubation with the NaK-ATPase and HK-ATPase produced partial (> 50%) inactivation, but had no effect on myosin Sl, pyruvate kinase and hexokinase, suggesting that this ATP analog is a specific inactivator of the so-called ’quo;P-type’ ATPases.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] ATPase;Affinity label;Carbodiimide;ATP derivative;SR sarcoplasmic reticulum;Mops 3-(N-morpholino)propanesulionic acid;TEOA;triethanolamine;EGTA;ethylene glycol bis(2-aminoethyl ether) N;N;N';N'-tetraacetic acid;AMP-PNP;adenyl-5'-ylimidodiphosphate;AMP-PCP;adenyl-5'-ylmethylenediphosphonate;TNP-ADP;2';3'-(2;4;6-trinitro-cyclohexadienylidene) adenosine 5'-diphosphate;EDC;1-ethyl-3-(3-diiriethylaminopropyl)-carbodiimide hydrochloride;EP;phosphorylated enzyme intermediate;ATP-EDC;αmma;Padduct of ATP and EDC;PK;pyruvate kinase;LDH;lactate dehydrogenase;PEP;phosphoenol pyruvate [时效性]
