Interferon‐γ activates the cleavage of double‐stranded RNA by bovine seminal ribonuclease

[摘要]

Bovine seminal ribonuclease (BS-RNase), a dimeric homologue of RNase A, cleaves both single- and double-stranded RNA and inhibits the growth of tumor cells. Its catalytic activity against double-stranded RNA, either homopolymeric ([³H]polyA/polyU) or mixed sequence, is enhanced by bovine or human recombinant interferon-γ (IFN-γ). Activation is seen with as little as 4–10 interferon units per assay. Enhancing the degradation of double-stranded RNA, an intermediate in the growth cycle of many viruses, could contribute to IFN-γ's ability to control cell growth and induce an antiviral state.

[学科分类] 生物化学/生物物理

[关键词] Interferon-γ Interferon-α;Ribonuclease A (RNase A);Bovine seminal ribonuclease;Mechanism of cell growth inhibition by interferons;Activation of double-stranded RNA degradation;IFN-γ;interferon-γ;IFN-α';interferon-α;hu;human;bo;bovine;RNase A;ribonuclease A;BS-RNase;bovine seminal ribonuclease;IFU;interferon units;ss- or ds-RNA;single-stranded or double-stranded RNA;TCA;trichloroacetic acid