We have expressed rat protein kinase C-γ in insect cells using a baculovirus vector. The yield of expressed protein kinase C-γ is about 4% of total protein. The recombinant protein shows a prominent band at about 80 kDa on SDS-polyacrylamide gels, which can be identified as protein kinase C-γ by Western blotting using monoclonal antibodies against protein kinase C-γ. Upon incubation with [γ-32P]ATP and in the presence of Ca2+ phosphatidylserine and diacylglycerol this protein autophosphorylates. Its enzyme activity shows the characteristic properties of mammalian protein kinase C.
