Firefly luciferase synthesizes P1,P4‐bis(5'‐adenosyl)tetraphosphate (Ap4A) and other dinucleoside polyphosphates

[摘要]

The synthesis of P¹,P⁴-bis(5'-adenosyl)tetraphosphate (Ap₄A) has been considered, for a long time, to be catalyzed mainly by some aminoacyl-tRNA synthetases [Brevet et al. (1989) Proc. Natl. Acad. Sci. USA 86, 8275-8279]. Recently, yeast Ap₄A phosphorylase, acting in reverse (Guranowski et al. (1988) Biochemistry 27, 2959-2964), was shown to synthesize Ap₄A, too. In the case of the synthetases, the intermediate complex E-aminoacyl-AMP may serve as donor of AMP to ATP, yielding Ap₄A. Here we demonstrate that firefly luciferase (EC 1.13.12.7) which forms the E-luciferin-AMP intermediate also synthesizes Ap₄A as well as other dinucleoside polyphosphates. We suggest moreover that: other enzymes (mainly synthetases and some transferases), which catalyze the transfer of a nucleotidyl moiety, via nucleotidyl-containing intermediates and releasing PP_i may produce dinucleoside polyphosphates.

[学科分类] 生物化学/生物物理

[关键词] Diadenosine tetraphosphate;Dinucleoside polyphosphate;Firefly luciferase;Pyrophosphate;Synthetase;Ap4A;diadenosine 5';5'''-P1;P4-tetraphosphate or diadenosine tetraphosphate;Ap4G;adenosine 5'-P1-tetraphosphoP4-5'''-guanosine;Gp4G;diguanosine 5';5'''-P1;P4-tetraphosphate or diguanosine tetraphosphate;Np4N;dinucleoside tetraphosphate;DNPP;dinucleoside polyphosphate;NMP;nucleoside monophosphate;X;organic acid able to form an anhydride bond with NMP