The role of phospholipase A2 in lipid peroxidation‐induced fall of membrane potential of rat liver mitochondria

[摘要]

Cumene hydroperoxide (230 μM)-induced fall of the membrane potential takes place only in Ca²⁺ -loaded mitochondria. Inhibitor of phospholipase A₂, P-bromphenacyl bromide prevents uncoupling of mitochondria, having no effect on the accumulation of lipid peroxidation products.

[学科分类] 生物化学/生物物理

[关键词] Mitochondria;Lipid peroxidation;Calcium;Phospholipase A2;CuOOH;cumene hydroperoxide;TPP+ tetraphenylphosphonium;MDA;malonic dialdehyde;TBA;2-thiobarbituric acid;Δφ;mitochondrial inner membrane potential;P-BrPhBr;P-bromphenacyl bromide;CCCP;carbonyl cyanide m-chlorphenyl-hydrazone