Characterization of the vir A gene of the agropine‐type plasmid pRiA4 of Agrobacterium rhizogenes

[摘要]

We sequenced a 4.2-kb DNA region encompassing the virA locus of the hairy-root-inducing plasmid pRiA4, and compared its sequence with the published virA region sequences of four tumor-inducing plasmids. An open reading frame capable of coding for 829 amino acids was identified for virA. Deletion mutants of virA constructed by fusing to lacZ, but not the wild-type gene itself, were efficiently expressed in Escherichia coli when they were put downstream front the lac promoter. These fused gene products became soluble or insoluble depending on the length of their lacZ moieties.

[学科分类] 生物化学/生物物理

[关键词] Agrobacterium;Agropine;Plasmid evolution;Signal transduction;Transcriptional regulation: Virulence gene;bp;base-pair(s);IPTG;ispropyl-β-D-thiogalacto-pyranoside;kb;kilobase or 1000 bp;nt;nucleotide position(s) corresponding to the numbers in Fig. 2;ORF;open reading frame;pRihairy-root-inducing plasmid;pTi;tumor-inducing plasmid;SDS;sodium dodecyl sulfate;vir;virulence gene(s);VirA;protein coded for by virA;VirG;protein coded for by virG