The α2 B adrenergic receptor of undifferentiated neuroblastoma × glioma hybrid NG108‐15 cells, interacts directly with the guanine nucleotide binding protein, Gi2

[摘要]

In membranes of undifferentiated neuroblastoma × glioma hybrid cell line NG108-15, the apparent specific binding of [³H]yohimbine measured in the presence of 1 μM noradrenaline, was increased substantially by the presence of the poorly hydrolysed analogue of QTP, guanylyl-imidodiphosphate (Gpp[NH]p) or by preincubation of membranes with antibodies against the C-terminal decapeptide of the α subunit of the G-protein G_i2. Such an effect was not produced by antibodies against the equivalent region of G_oα G_i3α or G_sα or from non-immune serum. By contrast, total specific binding of [³H]yohimbine was not modified by co-incubation with Gpp[NH]p or by preincubation with the antibodies from any of the anti-G protein antisera. These results demonstrate a direct interaction of the α₂B adrenergic receptor of NG108-15 cells with G_i2.

[学科分类] 生物化学/生物物理

[关键词] α2B Adrenergic receptor;Guanine nucleotide binding protein;Anti-peptide antiserum;Pertussis toxin;G-protein;guanine nucleotide binding protein;Gpp[NH]p;guanylyl-imidodiphosphate;IC50;concentration of ligand causing 50% inhibition of specific receptor binding