(+)‐Tubocurarine is a potent inhibitor of cation channels in the vacuolar membrane of Chenopodium rubrum L

[摘要]

The effect of the acetylcholine antagonist and channel blocker (+)-tubocurarine on the calcium-dependent slow vacuolar (SV) cation channels in the tonoplast of suspension-cultured cells of Chenopodium rubrum L. was examined using the patch-clamp technique. In whole-vacuolar recordings the drug strongly suppressed the potassium conductance (EC₅₀: 6 μM) and altered the kinetics of channel inactivation. In excised membrane patches (+)-tuboeurarine evokes channel-‘flickering’ without affecting the single-channel conductance (approx. 80 pS).

[学科分类] 生物化学/生物物理

[关键词] Patch-clamp technique;Plant vacuole;Potassium channel;Tonoplast;(+)-Tubocurarine;Chenopodium rubrum L