Determination of tRNA nucleotide residues directly interacting with proteins in the post‐ and pretranslocated ribosomal complexes

[摘要]

Nucleotide residues of E.coli tRNA interacting directly with proteins in pre- and posttranslocated ribosomal complexes have been identified by analysis of photo-induced tRNA-protein cross-links. A9, G 18, A26 and U59 residues of NAcPhePhe-tRNA, located in the A_b-site (pretranslocated complex) have been cross-linked with proteins S10, L27, S7 and L2 respectively. In deacylated tRNA, located in the P_b-site, residues C17, G44, C56 and U60 have been cross-linked with proteins L2, L5, L27 and S9 respectively. The G44-L5 cross-link disappeared after translocation (NAc-PhePhe-tRNA located in the P_t-site).

[学科分类] 生物化学/生物物理

[关键词] Escherichia coli ribosome;tRNA-binding site;tRNA-protein contacts;UV-induced tRNA-protein cross-links;Localization of cross-linked tRNA residues