Expression of subunit III ot the ATP synthase from spinach chloroplasts in Escherichia coli

[摘要]

Expression of subunit III of the ATP synthase from spinach chloroplasts in Escherichia coli has been achieved. Although the protein is inserted into the bacterial cytoplasmic membrane, formation of a functional F_o complex was not observed.

[学科分类] 生物化学/生物物理

[关键词] F1Fo;ATP synthase;Fo complex;Proteolipid;Complementation;DCCD;N.N'-dicyclohexylcarbodiimide;SDS;sodium dodecyl sulfate;IPTG;isopropyl β-D-thiogalactopyranoside