Involvement of activator protein in the activation of tryptophan hydroxylase by cAMP‐dependent protein kinase

[摘要]

The tryptophan hydroxylase activity of the crude extract from rat brain stem was stimulated approximately 2-fold by incubation with cAMP analogues under protein phosphorylating conditions. The cAMP-dependent activation process of the enzyme needed not only cAMP-dependent protein kinase but also activator protein. The kinetic properties of the enzyme activated by cAMP-dependent protein kinase were very similar to those of the enzyme activated by calmodulin-dependent protein kinase II.

[学科分类] 生物化学/生物物理

[关键词] Tryptophan hydroxylase;cAMP-dependent protein kinase;Calmodulin-dependent protein kinase II;Activator protein;14-3-3 protein;Rat brain;PKA;cAMP-dependent protein kinase;CaM-kinase II;calmodulin-dependent protein kinase II;Mes;4-morpholine-ethanesulfonic acid;Hepes;4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;NSD-1015;3-hydroxybenzylhydrazine;6MPH4;2-amino-4-hydroxy-6-methyltetrahydropteridine;8Br-cAMP;8-bromoadenosine 3';5'-cyclic monophosphate;db-cAMP;N 6;2'-O-dibutyryladenosine 3';5'-cyclic monophosphate