Protein kinase C during differentiation of human promyelocytic leukemia cell line, HL‐60

[摘要]

Protein kinase C (PKC) from human promyelocytic leukemia HL-60 cells can be resolved into three fractions (peak a, b and c) by hydroxyapatite column chromatography. Peak a and c enzymes are indistinguishable from the brain type II PKC having β (βI and βII)-sequence and type III having α-sequence, respectively. Peak b enzyme is a previously unidentified PKC subspecies that has enzymological properties subtly different from type I (having γ-sequence), type II and type III PKC. Upon treatment of HL-60 cells with 1 μM retinoic add, this peak b enzyme is decreased dramatically within 24 h, whilst peak a enzyme (β-PKC) is increased, and peak c (α-PKC) enzyme is slightly decreased within 48 h. The result implies that the PKC subspecies in HL-60 cells have distinct functions during cell differentiation.

[学科分类] 生物化学/生物物理

[关键词] Protein kinase C;Retinoic acid;Differentiation;(HL-60 cells);PKC;protein kinase C;RA;retinoic acid;DMSO;dimethyl sulfoxide;TPA;12-O-tetradecanoylphorbol-13-acetate;PS;phosphatidylserine;DO diolein;EGTA [ethylenebis(oxyethylenenitrilo)]tetraacetic acid