Reversal by EGTA of the enhanced secretory responsiveness of mast cells due to treatment with ouabain

[摘要]

The effect of EGTA on the enhancement by ouabain of compound -induced secretion from mast cells was compared with the effect on the Na⁺-K⁺ pump activity. The time-dependent secretory enhancement by ouabain was blocked by addition of EGTA to the cell suspension concomitantly with the addition of ouabain, and EGTA caused a large increase in the pump activity. Addition of 10 μM EGTA to ouabain-treated cells stopped but did not reverse the enhancement. The experiments show that the effect of ouabain was due to changes in a calcium pool utilized in compound -induced secretion following changes in the Na⁺,K⁺ pump activity.

[学科分类] 生物化学/生物物理

[关键词] Ouabain;Mast cell;EGTA;Chelating agent;ATPase;Na+;K+-;Ca2+