Identification of a Gs‐protein coupling domain to the β‐aderenoceptor using site‐specific synthetic peptides

[摘要]

Competition between G_s-protein and the synthetic peptide, GSA 379-394, derived from the carboxyl-terminal region of the α_s-subunit, led to complete inhibition of receptor-mediated adenylate cyclase activation in turkey erythrocyte membranes. Related peptides corresponding to the homologous carboxyl-terminal region of α_t-,α_il- or α_o-subunits did not interfere with β-receptor-G_s coupling. The direct coupling between G_s and adenylate cyclase was not influenced by any of these peptides. These results emphasize the important role of the carboxyl-terminus of G-protein α-subunits for the specific recognition of their corresponding receptors and for signal transduction.

[学科分类] 生物化学/生物物理

[关键词] Adrenoceptor;β-;Gs-protein;Coupling domain;Synthetic peptide;Anti-peptide antibody;Gs-protein;guanine nucleotide-binding protein mediating stimulation of adenylate cyclase;αt;αo and αil;α-subunits of corresponding G-proteins;C;adenylate cyclase;GTPγS;guanosine-5'-O-(3-thiotriphosphate);TFA;trifluoroacetic acid