Fluorescence properties of the Ca2+Mg2+‐ATPase protein of sarcoplasmic reticulum labeled with 7‐chloro‐4‐nitrobenzo‐2‐oxa‐1,3‐ diazole

[摘要]

The fluorescence intensity of the Ca²⁺,Mg²⁺-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 μM calcium. The observed enhancement indicates that the fluorophore, NBD-Cl, can detect conformational changes in the ATPase protein.

[学科分类] 生物化学/生物物理

[关键词] Sarcoplasmic reticulum;Skeletal muscle;ATPase;Fluorescence labeling