Sequencing of potato mitochondrial (mt) tRNALeu(NAA) and of its cytosolic (cyt) counterpart revealed that these tRNAs are identical, except for a post-transcriptional modification: a Gm is present at position 18 in mt tRNALeu, instead of a G in cyt tRNALeu. Hybridization studies have shown that potato mt tRNALeu(NAA) has a nuclear origin and must therefore be imported from the cytosol.