Evidence that chlorpromazine and prostaglandin E1 but not neomycin interfere with the inositol phospholipid metabolism in intact human platelets

[摘要]

Human platelets that had been prelabelled with [³²P]P_i were stimulated with trombin in the presence or absence of neomycin, prostaglandin E₁ (PGE₁) or chlorpromazine. The content of [³²P]P_i in phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidic acid (PA) were determined. The data demonstrate that PGE₁ and chlorpromazine but not neomycin interfere with the tight metabolic relationship that exists between the inositol phospholipids and PA in thrombin-stimulated platelets [(1989) Biochem. J. 263, 621-624]. Our results therefore indicate that neomycin does not inhibit signal transduction in intact platelets at the level of the inositol phospholipid metabolism.

[学科分类] 生物化学/生物物理

[关键词] Neomycin;Chlorpromazine;Prostaglandin E1;Signal transduction;Inositol phospholipid;Thrombin;Platelet;PA;phosphatidic acid;PIP;phosphatidylinositol 4-phosphate;PIP2;phosphatidylinositol 4;5-bisphosphate;PGE1;prostaglandin E1;PPI;polyphosphoinositide