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Two‐dimensional crystallization of reaction centers from Chloroflexus aurantiacus
[摘要]

Two-dimensional crystals of photosynthetic reaction centers from Chloroflexus aurantiacus were obtained from protein-lipid-detergent micelles by detergent dialysis. The size of crystals was up to 2 μm. Some of them were multilayered crystals. However, other crystal forms were also observed. Preliminary image processing analysis showed that crystals of one crystal form referred to two-sided plane group p2 and had the following unit cell parameters: a = 17,6 nm, b = 18,0 nm, γ = 84°. The contour map of the crystal stain-excluding region was calculated by the Fourier-filtering procedure at about 2 nm resolution.

[发布日期]  [发布机构] 
[效力级别]  [学科分类] 生物化学/生物物理
[关键词] Reaction center;Two-dimensional crystal;Electron microscopy;Image processing;LDAO;lauryldimethylamine N-oxide;RC;reaction center [时效性] 
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