Changes of fluorescence anisotropy in plasma membrane of human polymorphonuclear leukocytes during the respiratory burst phenomenon

[摘要]

Steady state fluorescence anisotropy (r _s) of TMA-DPH was measured to study the effect of respiratory burst activation with PMA, FMLP, and PAF on the physico-chemical structure of PMNs plasma membrane. Our results show a significant increase in r _s during the respiratory burst activation. In the presence of NADPH-oxidase inhibitor DPI, only PAF induces changes in r _s values. This suggests a non-specific effect of PAF on plasma membrane. Azide, which induces a supranormal release of H₂O₂, fails to increase the basal r _s value after activation. Moreover, the catalase does not abolish the increase in rr _s induced upon activation. This rules out the possibility that changes of r _s during the respiratory burst activation are attributed mainly to H₂O₂ release. We conclude that multiple processes accompanying the respiratory burst activation are responsible for the changes in the physico-chemical properties of PMNs plasma membrane.

[学科分类] 生物化学/生物物理

[关键词] Fluorescence anisotropy;TMA-DPH;Respiratory burst;PMN;PMNs;polymorphonuclear leukocytes;TMA-DPH;1-(4-trimethylammoniumphenyl)-6-phenyl-1;3;5-hexatriene;PMA;phorbol myristate acetate;FMLP;N-formyl-methionyl-leucyl-phenylalanine;PAF;1-alkyl-2-acetyl-sn-glycero-3-phosphocholine;DPI;diphenyl iodonium