Formylmethanofuran: Tetrahydromethanopterin formyltransferase from Methanosarcina barkeri Identification of N 5‐formyltetrahydromethanopterin as the product

[摘要]

Formylmethanofuran: tetrahydromethanopterin formyltransferase was purified from methanol grown Methanosarcina barkeri to apparent homogeneity and characterized with respect to its molecular and kinetic properties. The enzyme was found to be very similar to the formyltransferase from H₂/CO₂ grown Methanobacterium thermoautotrophicum. It also catalyzed the formation of N ⁵-formyltetrahydromethanopterin rather than of N ¹⁰-formyltetrahydromethanopterin from formylmethanofuran and tetrahydromethanopterin.

[学科分类] 生物化学/生物物理

[关键词] Methanofuran;Formylmethanofuran;Tetrahydromethanopterin;Formyltetrahydromethanopterin;Methanosarcina barkeri;Methanobacterium thermoautotrophicum;MFR;methanofuran;H4MPT;tetrahydromethanopterin;MES;β-morpholinoethane sulfonic acid;MOPS;γ-morpholinopropane sulfonic acid;Tricine;N-[Tris(hydroxymethyl)methyl]glycine;1 U;1 μmol/min