Species‐specific Mn2+/Mg2+ antiport from Mg2+‐loaded erythrocytes

[摘要]

Mg²⁺-loaded rat erythrocytes performed Mn⁺/Mg²⁺ antiport, which was nonspecifically stimulated by anions and cations. Mn²⁺/Mg²⁺ antiport was shown to operate via the Na⁺Mg²⁺ antiporter because extracellular Na⁺ and Mn²⁺ inhibited the intracellular uptake of each other's ions competitively. Furthermore, Mn²⁺/Mg²⁺ antiport and Na⁺/Mg²⁺ antiport were identically inhibited by various amiloride derivatives. Na⁺/Mg²⁺ antiport of chicken and human erythrocytes cannot perform Mn²⁺/Mg²⁺ antiport although chicken erythrocytes took up more Mn²⁺ than raterythrocytes.

[学科分类] 生物化学/生物物理

[关键词] Mn2+ uptake;Mg2+ efflux;(Rat erythrocyte);[Na+]0;[Cl−]0;[Mn2+]0;extracellular concentration of Na+;Cl−;or Mn2+;TCA;trichloroacetic acid;SITS;4-acetamido-4' -isothiocyanatostilbene-2;2' -disulfonic acid