Crystallisation and preliminary X‐ray diffraction studies of cyclophilin‐tetrapeptide and cyclophilin‐cyclosporin complexes

[摘要]

Recombinant human cyclophilin has been co-crystallised with a number of peptides to give crystals suitable for X-ray analysis. The crystal complexes for which heavy-atom derivatives have been prepared and X-ray data collected are: cyclophilin with N-acetyl-Ala-Ala-Pro-Ala-amidomethylcoumarin (I) which crystallises in space group P2₁2₁2₁ with a = 108.2, b = 123.0, c = 35.8 Å, and cyclophilin with cyclosporin (II) which crystallises as tetragonal plates in space group P4₁2₁2 or P4₃2₁2 with a = b = 94.98, c = 278.55 Å.

[学科分类] 生物化学/生物物理

[关键词] Cyclophilin;Cyclosporin;Protein crystallisation;Cis-trans isomerase;Linear dichroism