Immunocytochemical evidence of vertebrate bioactive peptide‐like molecules in the immuno cell types of the freshwater snail Plianorbarius corneus (L.) (Gastropoda, Pulmonata)

[摘要]

An immunocytochemical investigation was carried out on round and spreading hemocytes of Planorbarius comeus by using 20 antisera to vertebrate bioactive peptides. The immunotests showed the presence of α₁antichymotrypsin-bombesin-, calcitonin-, CCK-8 (INC)-, CCK-39-, gastrin-, glucagon-, Met-enkephalin-, neurotensin-, oxytocin-, somatostatin-, substance P-, VIP-, and vasopressin-immunoreactive molecules in the spreading hemocytes. The round hemocytes were only positive to anti-bombesin, anticalcitonin, anti-CCK-8 (INC), anti-CCK-39, anti-neurotensin, anti-oxytocin, anti-substance P and anti-vasopressin antibodies. No immunostaining was observed with anti-CCK-8 (Peninsula), anti-insulin, anti-prolactin, anti-thyroglobulin and anti-thyroxin (T4) antibodies. As probably in vertebrates, these bioactive peptides may modulate immuno cell function.

[学科分类] 生物化学/生物物理

[关键词] Planorbarius corneus;Hemocyte;Bioactive peptide;Immunocytochemistry