Modification methylase M.Sau3239I from Streptomyces aureofaciens 3239

[摘要]

By chromatography on phosphocellulose and Heparin-Sepharose the modification methylase M.Sau3239I was detected and partly purified from cells of Streptomyces aureofaciens 3239. Methylation by this enzyme protects DNA from cleavage by the restriction endonuclease R.Sau3239I. The enzyme catalyzes methylation of adenine to N-6-methyladenine in the 5'-CTCG^mAG-3' recognition sequence.

[学科分类] 生物化学/生物物理

[关键词] Streptomyces aureofaciens;DNA methylation;Modification methylase