Human tRNAGlu genes: their copy number and organisation

[摘要]

The tRNA^Glu gene copy number, determined by genomic blot analysis of human plaeental DNA, is approximately thirteen. These studies, using several probes and DNA digested with several restriction enzymes singly or in combination, show that most of these tRNA^Glu genes are flanked by DNA of very similar sequence for at least 5 kb. This conclusion is supported by the close similarity of the restriction maps of two λ Charon-4A recombinants of human genomic DNA containing two different tRNA^glu genes.

[学科分类] 生物化学/生物物理

[关键词] Recombinant DNA;tRNAGlu gene;λ Charon4A;Copy number;Genomic blot;Multigene family;bp;base pair(s);kb;kilobases or 1000 bp;tRNA;transfer RNA;nt(s);nucleotides;p;plasmid;SDS;sodium dodecyi sulphate