The platelet‐derived growth factor isomers, PDGF‐AA, PDGF‐AB and PDGF‐BB, induce contraction of vascular smooth muscle cells by different intracellular mechanisms

[摘要]

The effect of human recombinant platelet-derived growth factor (PDGF) isoforms, (r)PDGF-AA, PDGF-AB and PDGF-BB, on contractility of rat aortic rings as well as on intercellular free Ca²⁺ ([Ca²⁺]_i), intracellular pH_i (pH_i) and thromboxane A₂ (TXA₂) formation in cultured vascular smooth muscle cells (VSMC) was examined. PDGF-BB behaved similar to PDGF-AB and both have features characteristic of conventional vasocontrictor-agonists that directly increase [Ca²⁺]_i, activate the Na⁺/H⁺ exchanger, stimulate the TXA₂ formation, and induced contraction in VSMC whereas PDGF-AA induced contraction without increasing of [Ca²⁺]_i, pH_i, and TXA₂ formation.

[学科分类] 生物化学/生物物理

[关键词] PDGF-isoform;Vasoconstriction;Intracellular Ca2+ and pH;Thromboxane A2;[Ca2+]i;intracellular free Ca2+;pHi;intracellular pH;TXA2;thromboxane A2;VSMC;vascular smooth muscle cells;PDGF;platelet-derived growth factor;BCECF;2′;7′-bis(carboxyethyl)-5;6-carboxyfluorescein;PBS;phosphate-buffered saline;Hepes;4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid