We studied binding and growth inhibitory properties of different glycosaminoglycans in growing and differentiated BC3H-1 muscle cells. Heparin (10 80723-V/asset/equation/feb2001457939080723v-math-si1.gif?v=1&s=ecca0a24d5d31893eb99ab349b121601ae8dc02b)
) and heparan sulfate (10 80723-V/asset/equation/feb2001457939080723v-math-si2.gif?v=1&s=27c840949933543ae54350cec5e5187e3a31908a)
) significantly inhibited DNA synthesis in growing and differentiated cells, as monitored by [3H] thymidine incorporation. Binding of heparin to BC3H-1 cells was specific and time-dependent. Heparan sulfate was the only glycosaminoglycan able to displace [3H]heparin (IC50, 3.2 × 10−7 M), although it was 10-fold less effective than heparin itself (IC50 3.6 × 1O−8 M). Scatchard analysis revealed the existence of high-affinity hepariy binding sites (Kd, 5 × 10−8 M). Furthermore, heparin inhibited serum-induced stimulation of inositol lipid turnover. Taken together, these results indicate that heparin inhibits BC3H-1 cell growth by interacting with the cell surface, possibly disrupting the flow of growth factor-related mitogenic signalling.
