Solubilization of sarcoplasmic reticulum membranes by sodium dodecylsulphate

[摘要]

In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca²⁺-ATPase activity, membrane protein solubilization, and protein conformation have been independently monitored, and correlations among the various parameters have been observed. The thermal denaturation of sarcoplasmic reticulum proteins in the presence of sodium dodecylsulphate has also been characterized spectroscopically.

[学科分类] 生物化学/生物物理

[关键词] Membrane solubilization;Protein denaturation;Sarcoplasmic reticulum Ca2+-ATPase;Surfactant;Fourier-transform infrared spectroscopy;D/P;detergent:protein mole ratio;FT-IR;Fourier-transform infrared;SDS;sodium dodecylsulphate;SR;sarcoplasmic reticulum