Enzymatic synthesis of nucleoside 5′‐mono and ‐triphosphates

[摘要]

The transformation of fluorodeoxy-nucleosides into 5'-monophosphates with the use of whole bacterial cells of Erwinia herbicola as a biocatalyst and PNP as a phosphate donor and next into 5'-triphosphates by using an extract of the Saccharomyces cerevisiae cells has been demonstrated.

[学科分类] 生物化学/生物物理

[关键词] Nucleoside 5'-monophosphate;Nucleoside phosphotransferase;Nucleoside 5'-triphosphate;Nucleotide kinase;(Saccharomyces cerevisiae;Erwinia herbicola);NTP(3'F);3'-fluoro-3'-deoxyribunocleoside 5'-tri-phosphates with the bases adenine (ATP(3'F)];guanine [GTP(3'F)];uracil [UTP(3'F)] and cytosine [CTP(3'F)];NMP(3'F);3'-fluoro-3'-deoxyribonucleoside 5'-monophosphates with the natural bases;Ado(3'F);Guo(3'F);Urd(3'F);and Cyt(3'F);3'-fluoro-3'-deoxyribonucleosides;dNTP(3'F) and dNMP(3'F);3'-fluoro-2';3'-di-deoxyribonucleoside 5'-tri- and -monophosphates;respectively;dAdo (3'F);dGuo(3'F);dThd(3'F) and dCyd(3'F);3'-fluoro-2';3'-dideoxyribonueleosides;PNP;p-nitrophenylphosphate;NMP and NTP;nucleoside 5'-mono- and -triphosphates;respectively