Carnitine palmitoyltransferase (CPT2) from liver mitochondrial inner membrane becomes inhibitable by malonyl‐CoA if reconstituted with outer membrane malonyl‐CoA binding protein

[摘要]

A soluble extract was obtained on treatment of rat liver mitochondrial outer membranes with cholate which bound (¹⁴C]malonyl-CoA but was essentially free of carnitine palmitoyltransferase (CPT) activity. Extraction of mitochondrial inner membranes with cholate readily solubilized a CPT activity which was insensitive to malonyl-CoA. Combination of these two extracts caused the CPT derived from inner membranes to become inhibitable by malonyl-CoA.

[学科分类] 生物化学/生物物理

[关键词] Carnitine palmitoyltransferase;Malonyl-CoA;Liver;Mitochondria