The cosubstrate NADP(H) protects lysine 601 in the porcine NADPH‐cytochrome P‐450 reductase against pyridoxylation

[摘要]

Lys⁶⁰¹ in NADPH-cytochrome P-450 reductase is modified by reductive alkylation with pyridoxal 5'-phosphate (pyridoxylation). Lys⁶⁰¹ is protected against modification by the cosubstrate NADP(H).

[学科分类] 生物化学/生物物理

[关键词] NADP(H) binding site;Lysine residue;Pyridoxal 5'-phosphate;Micro sequencing;(Pig liver);P450R;NADPH-cytochrome;P-450 reductase protease-solubilized (EC 1.6.2.4);NADP-PPi;NADP pyrophosphoryl binding site;(CN)3 P450R;NADPH-cytochrome P-450 reductase S-cyanylated at the accessible cysteines;PALP;pyridoxal s'-phosphate;pyridoxylation;substitution of lysyl residues by reductive alkylation with PALP;DTNB;5;5'-dithiobis(2-nitrobenzoate);TNB−;2-nitro-5-thiobenzoate