Evidence that the putative α‐glucosidase of Thermotoga maritima MSB8 is a pNP α‐D‐glucuronopyranoside hydrolyzing α‐glucuronidase

[摘要]

The gene (agu) encoding p-nitrophenyl α-D-glucuronopyranoside (pNP-GUA) hydrolyzing α-glucuronidase of the hyperthermophilic bacterium Thermotoga maritima was cloned and expressed in Escherichia coli. The recombinant enzyme was purified and characterized. The gene previously designated as putative α-glucosidase was found to code for a protein that had no α-glucosidase activity. It showed a rare activity profile with its ability to hydrolyze pNP-GUA, an activity not known in the α-glucuronidases from microbial sources. This is the first report on the occurrence of an α-glucuronidase which belongs to the family 4 of glycosyl hydrolases.

[学科分类] 生物化学/生物物理

[关键词] Hyperthermophile;p-Nitrophenyl α-D-glucuronopyranoside hydrolyzing α-glucuronidase;Family 4 glycosyl hydrolase;Thermotoga maritima;pNP;p-nitrophenol;pNP-GUA;p-nitrophenyl α-D-glucuronopyranoside;pNP-Glc;p-nitrophenyl α-D-glucopyranoside;pNP-G6P;p-nitrophenyl α-glucopyranoside-6-phosphate;DTT;dithiothreitol