Phosphorylation of the regulatory subunit of smooth muscle protein phosphatase 1M at Thr850 induces its dissociation from myosin

[摘要]

Rho kinase is known to control smooth muscle contractility by phosphorylating the 110 kDa myosin-targetting subunit (MYPT1) of the myosin-associated form of protein phosphatase 1 (PP1M). Phosphorylation of MYPT1 at Thr695 has previously been reported to inhibit the catalytic activity of PP1. Here, we show that the phosphorylation of Thr850 by Rho kinase dissociates PP1M from myosin, providing a second mechanism by which myosin phosphatase activity is inhibited.

[学科分类] 生物化学/生物物理

[关键词] Protein phosphatase 1;Rho kinase;Smooth muscle;Myosin;PP1;protein phosphatase 1;PP1M;smooth muscle protein phosphatase 1;PP1C;protein phosphatase 1 catalytic subunit;MYPT1;myosin phosphatase targetting 1;ROKα/ROCK-II;Rho-dependent protein kinase