Isolation of oxygen‐evolving phycobilisome—photosystem II particles from Porphyridium cruentum

[摘要]

Photosystem II phycobilisome particles were isolated from the red alga Porphyridium cruentum in a sucrose—phosphate—citrate—magnesium medium. These particles showed high rates of oxygen evolution in the water-to-silicomolybdate reaction (2300 μmol O₂.mg chl^-1.h^-1). Photosystem I, according to fluorescence emission spectra (−196°C), was greatly reduced or absent. The results demonstrate a close physical relationship of photosystem II with the phycobilisomes which are the major light harvesting antennae in this organism.

[学科分类] 生物化学/生物物理

[关键词] Phycobilisome;Photosystem II;Oxygen evolution;Light-harvesting antenna;(Porphyridium cruentum);(Red alga);SPCM;sucrose—phosphate—citrate—magnesium chloride medium;chl;chlorophyll;PS I;PS II;photosystem I and II;respectively;LDAO;lauryldimethylamine oxide;DMSO;dimethylsulfoxide;DMBQ;2;6-dimethyl-p-benzoquinone;SiMo;12-silicomolybdate (Na4Mo12O40);DCMU;3-(3;4-dichlorophenyl)-1;1 dimethylurea;NH2OH;hydroxylamine